Cryopreservation supplies and equipment

Cryopreservation Protocols Contents

Sperm

Embryo

Ovary

IVF

Media

Supplies and Equipment

 

This is list of supplies and equipment that we use in our laboratory and/or in teaching our courses. We have no financial interest in any of the vendors and manufacturers listed. Furthermore, we do not mean to imply that the material listed here is any better or worse than similar material available from other manufacturers.

I. Supplies

Item Catalog # Vendor
Cryo Tubes (Nunc) 12-565-170N Fisher Scientific Company

52 Fadem Road

Springfield, N.J. 07081

Culture dishes, 35 x 10mm Falcon 3001 Common scientific suppliers
Culture tubes, 12 x 75 mm 14-962-10B Fisher
Embryo flushing concavity dishes *   Morita Seisakujo

2-12-12 Yushima Bunkyo-ku

Tokyo, 113 Japan

Tel: 03-811-9730 Fax: 03-812-0326

Embryo flushing needle: 30 g 1/2 in   Common scientific suppliers
Embryo flushing syringe: 5 cc   Common scientific suppliers
Embryo pipettes (general): 4 mm o.d. pyrex tubing (cut into 6" lengths and drawn out over flame)   Common scientific suppliers
Embryo pipettes (transfer ): 100 ?l pipettes - Gold Seal Accu-Fill 90 Micropet (drawn out over small flame) 7707-B45 Arthur Thomas Scientific

99 High Hill Road

P.O. Box 99

Swedesboro, NJ 08085

Embryo pipette tubing (transfer ): 9555-N27 Arthur Thomas
Filter units for media sterilization 0.45?m CoStar 8363 Co-Star

205 Broadway

Cambridge, MA 02139

Filter units for oil sterilization 0.8?m Nalgene 450-0080 Common scientific suppliers
Micrometer head Mitutoyo Micrometer Head 150-208

(~$50)

Westco Industrial Distributors

145 Presumpscot St.

Portland, ME 04104

(207) 774-5812

Mineral oil (light) 33,077-9 Aldrich

1001 West Saint Paul Ave.

Milwaukee, WI 53233

Modular incubator chamber 190043 Forma Scientific

Box 649

Marietta, OH 45750

Pipette (bulk samples) Selectapette and 1 ml tips - Clay-Adams 21-260 pipette gun

21-262 pipette tips

Fisher
Pipette (micro): 100 ?l Eppendorf 21-370G Fisher
Pipette tips (micro): 100 ?l 4864 Co-Star
Surgical instruments: fine watch maker forceps Dumont #5 Roboz

1000 Conn Avenue N.W.

Washington, DC 20036

Surgical instruments: forceps, Serrefine forceps, and scissors   Arthur Thomas
Surgical instruments: wound clips and application tool 01-804-10 - clips

01-804 - applier

Fisher

* These used to be readily available in this country, but now our only known source is Morita Seisakujo in Japan. Inquire by e-mail to info@mysci.co.jp. Total cost for 20 slides was $280 (December 2001) which was the minimum order.

II. Cryogenic equipment:

Item Vendor Telephone
CryoMed 1010 programmable freezing controller Forma Scientific, Inc.

Box 649 Marietta, Ohio 45750

1-800-848-3080
Series P.C. controller Custom Biogenic Systems

5562 Ten Mile Road

Warren, MI 48091

(313) 754-2278
BioCool IV controlled rate freezer FTS Systems, Inc.

PO Box 158

Stone Ridge, New York 12484-0158

(914) 687-7664
Liquid nitrogen dewers

3 liter G-03763-50

1 liter G-03763-20

Cole Parmer Instrument Co.

7425 North Oak Park Avenue

Niles, IL 60714

Tel: 1-800-323-4340

Fax: (708) 647-9660

Liquid nitrogen storage containers Minnesota Valley Engineering (MVE)

8011 34th Avenue South, Suite 100

Bloomington, MN 55425-1636

Tel: 1-800-247-4446

Fax: (612) 853-9666

  Taylor-Wharton Cryogenics

PO Box 568

Theodore, AL 36590

Tel: 1-800-428-3304

or (205) 653-8680

Fax: (205) 653-2209